Poly(2-allylamidopropyl-2-oxazoline)-Based Hydrogels: From Accelerated Gelation Kinetics to In Vivo Compatibility in a Murine Subdermal Implant Model
journal contribution
posted on 2021-03-25, 17:33 authored by Tim R. Dargaville, Damien G. Harkin, Jong-Ryul Park, Amanda Cavalcanti, Eleonore C. L. Bolle, Flavia Medeiros Savi, Brooke L. Farrugia, Bryn D. Monnery, Yann Bernhard, Joachim F. R. Van Guyse, Annelore Podevyn, Richard HoogenboomA rapid
photo-curing system based on poly(2-ethyl-2-oxazoline-co-2-allylamidopropyl-2-oxazoline) and its in vivo compatibility are presented. The base polymer was synthesized from
the copolymerization of 2-ethyl-2-oxazoline (EtOx) and the methyl
ester containing 2-methoxycarboxypropyl-2-oxazoline (C3MestOx) followed by amidation with allylamine to yield a highly water-soluble
macromer. We showed that spherical hydrogels can be obtained by a
simple water-in-oil gelation method using thiol–ene coupling
and investigated the in vivo biocompatibility of
these hydrogel spheres in a 28-day murine subdermal model. For comparison,
hydrogel spheres prepared from poly(ethylene glycol) were also implanted.
Both materials displayed mild, yet typical foreign body responses
with little signs of fibrosis. This is the first report on the foreign
body response of a poly(2-oxazoline) hydrogel, which paves the way
for future investigations into how this highly tailorable class of
materials can be used for implantable hydrogel devices.
