posted on 2016-02-10, 00:00authored byAndreas S. Andersen, Hüsnü Aslan, Mingdong Dong, Xingyu Jiang, Duncan S. Sutherland
We studied podosome formation and
development in activated monocytes (THP1) at ICAM1 (intercellular
adhesion molecule 1) nanopatterns of circular and ring-shaped domains
and show that cellular binding to a preclustered ICAM1 nanopattern
requires ligand patches of at least 200 nm (corresponding to 14 or
more integrins). Podosome-like adhesion formation depends on the structure
of the ligand pattern under the developing podosome with larger single
domains promoting adhesion in a single patch and multiple smaller
domains allowing podosome formation by integration of at least 2 smaller
domains on either side of the podosome core. Maturation to rosette
structures and recruitment of proteases were only observed with macroscopic
ICAM1 presentation.