posted on 2024-01-31, 12:03authored byLin Tian, Ming Cao, Haorong Cheng, Yanfei Wang, Changchun He, Xinxin Shi, Tongxiang Li, Zhao Li
Rationally
designing highly catalytic and stable nanozymes for
metabolite monitoring is of great importance because of their huge
potential in early disease diagnosis. Herein, a novel nanozyme based
on hierarchically structured CuS/ZnS with a highly efficient peroxidase
(POD)-mimic capability was developed and synthesized for multiple
metabolite determination and recognition via the plasmon-stimulated
biosensor array strategy. The designed nanozyme can simultaneously
harvest plasmon triggered hot electron–hole pairs and generate
photothermal properties, leading to a sharply boosted POD-mimic capability
under 808 nm laser irradiation. Interestingly, because of the interaction
diversity of the metabolite with POD-like nanomaterials, the unique
inhibitory effect of metabolites on the POD-mimic activity could be
the signal response as the differentiation. Thus, utilizing TMB as
a typical chromogenic substrate in the addition of H2O2, the designed colorimetric biosensor array can produce diverse
fingerprints for the three vital metabolisms (cysteine (Cys), ascorbic
acid (AA), and glutathione (GSH)), which can be precisely identified
by principal component analysis (PCA). Notably, a distinct fingerprint
of a single metabolite with different levels and metabolite mixtures
is also achieved with a detection limit of 1 μM. Most importantly,
cell lysis could be effectively discriminated by the biosensor assay,
implying its great potential in clinical diagnosis.