pr1004345_si_001.pdf (1.22 MB)
Plasma Proteomic Analysis of Simian Immunodeficiency Virus Infection of Rhesus Macaques
journal contribution
posted on 2010-09-03, 00:00 authored by Jayme L. Wiederin, Robert M. Donahoe, James R. Anderson, Fang Yu, Howard S. Fox, Howard E. Gendelman, Pawel S. CiborowskiLentiviral replication in its target cells affects a delicate balance between cellular cofactors required for virus propagation and immunoregulation for host defense. To better elucidate cellular proteins linked to viral infection, we tested plasma from rhesus macaques infected with the simian immunodeficiency viral strain SIVsmm9, prior to, 10 days (acute), and 49 weeks (chronic) after viral infection. Changes in plasma protein content were measured by quantitative mass spectrometry by isobaric tags for absolute and relative quantitation (iTRAQ) methods. An 81 and 232% increase in SERPINA1 was seen during acute and chronic infection, respectively. Interestingly, gelsolin, vitamin D binding protein and histidine rich glycoprotein were decreased by 45% in acute conditions but returned to baseline during chronic infection. When compared to uninfected controls, a 48−103% increase in leucine rich alpha 2-glycoprotein, vitronectin, and ceruloplasmin was observed during chronic viral infection. Observed changes in plasma proteins expression likely represent a compensatory host response to persistent viral infection.
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plasma protein contentisobaric tagsvitamin D binding proteinmass spectrometry49 weeksObserved changesSERPINA 1target cellsinfection10 dayshost defensePlasma Proteomic AnalysisRhesus MacaquesLentiviral replicationplasma proteins expressionvirus propagationhost responseSimian Immunodeficiency Virus Infectionrhesus macaques
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