ol200809m_si_001.pdf (1.26 MB)
Plantazolicin A and B: Structure Elucidation of Ribosomally Synthesized Thiazole/Oxazole Peptides from Bacillus amyloliquefaciens FZB42
journal contribution
posted on 2011-06-17, 00:00 authored by Bahar Kalyon, Soleiman E. Helaly, Romy Scholz, Jonny Nachtigall, Joachim Vater, Rainer Borriss, Roderich D. SüssmuthThe structures of the ribosomally synthesized peptide antibiotics from Bacillus amyloliquefaciens FZB42, plantazolicin A and B, have been elucidated by high resolving ESI-MSMS, 2D 1H–13C-correlated NMR spectroscopy as well as 1H–15N-HMQC/1H–15N-HMBC NMR experiments. 15N-labeling prior to the experiments facilitated the structure determination, unveiling a hitherto unusual number of thiazoles and oxazoles formed from a linear 14mer precursor peptide. This finding further extends the number of known secondary metabolites from B. amyloliquefaciens and represents a new type of secondary metabolites from the genus Bacillus.