posted on 2023-12-01, 13:04authored byJiayi Sun, Biqi Tang, Chi-Tang Ho, Muwen Lu
Piperine
(PIP), a pungent alkaloid found in black pepper, has various
pharmacological effects by activating the transient receptor potential
vanilloid 1 (TRPV1) receptor. In this study, the regulating effect
of PIP on glucose metabolism and the underlying mechanism were examined
using an insulin-resistant cell model. Results showed that PIP alleviated
glucosamine (GlcN)-induced glucose metabolism disorder (from 59.19
± 1.90 to 88.36 ± 6.57%), restored cellular redox balance
(from 148.43 ± 3.52 to 110.47 ± 3.52%), improved mitochondrial
function (from 63.76 ± 4.87 to 85.98 ± 5.12%), and mitigated
circadian disruption in HepG2 cells via the mediation of circadian
clock gene Bmal1. After the knockdown of the Trpv1 gene, the modulating effect of PIP on Bmal1-mediated glucose metabolism was weakened, indicating that PIP alleviated Bmal1-involved insulin resistance and circadian misalignment
in a Trpv1-dependent manner in HepG2 cells.