posted on 2022-01-19, 15:08authored byXiaobo Feng, Liang Ma, Jie Lei, Qunle Ouyang, Yuxuan Zeng, Yue Luo, Xiaoguang Zhang, Yu Song, Gaocai Li, Lei Tan, Xiangmei Liu, Cao Yang
The
successful treatment of osteomyelitis remains a great challenge
in the field of orthopedics. The clinical method for treating refractory
bone infection requires a combination therapy of long-term systemic
antibiotics administration and surgical debridement. It is highly
desirable to develop an antibiotic-free, noninvasive, rapid strategy
to eradicate osteomyelitis. Herein, we fabricate a piezoelectric-enhanced
sonosensitizer that consists of a porphyrin-based hollow metal–organic
framework (HNTM), MoS2 nanosheets, and a red cell (RBC)
membrane. We find that the ultrasound (US)-induced piezoelectric polarization
of MoS2 can improve the charge transfer of HNTM at the
heterointerface of HNTM-MoS2, increasing the production
of reactive oxygen species (ROS). Besides, MoS2 increases
the asymmetric shape of HNTM, leading to the strong US-propelling
ability of HNTM–MoS2. The produced ROS and strong
mechanical force can kill methicillin-resistant Staphylococcus
aureus (MRSA) with an antibacterial efficiency of 98.5% under
15 min of US treatment, resulting in intracellular DNA damage and
increased oxidative stress and disturbance of purine metabolism, tryptophan
metabolism, and pantothenate and CoA biosynthesis of MRSA. Together
with the toxin neutralization ability, the RBC-HNTM-MoS2 successfully eliminates the bone infection and suppresses inflammation
and bone loss. This work provides another strategy for developing
an efficient sonosensitizer through piezoelectric-assisted sonocatalysis
and enhancing US-propelling ability.