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Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress

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journal contribution
posted on 06.08.2015, 00:00 by A. C. Cutró, E. A. Disalvo
We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25 °C) and in liquid crystalline states (50 °C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does not produce significant changes in MC 540 monomer concentration in DPPC LUVs at 50 °C. In contrast, it significantly decreases the monomer adsorption in defects present in DPPC LUVs at 25 °C. When DPPC LUVs were subjected to hypertonic stress, dehydration caused more defects, and in this case phenylalanine is also able to block such defects.

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