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Download filePhage Display-Derived Peptide for the Specific Binding of SARS-CoV‑2
journal contribution
posted on 29.12.2021, 18:13 authored by Fan Yang, Li Liu, Pierre Fernand Neuenschwander, Steven Idell, Ramakrishna Vankayalapati, Krishan Gopal Jain, Ke Du, Honglong Ji, Guohua YiBeginning from the
end of 2019, the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) pandemic swept all over the world and is
still afflicting the whole global population. Given that the vaccine-manufacturing
ability is limited and the virus can evolve quickly, vaccination alone
may not be able to end the pandemic, thus developing fast and accurate
diagnoses and effective therapeutics will always be unmet needs. Phage
display peptide library has been used in screening antigen-specific
peptides for the invention of novel mimic receptors/ligands. Here,
we report that a 12-mer phage display peptide library has been screened
against the SARS-CoV-2 receptor-binding domain (RBD), and five of
the screened peptides show binding ability with the RBD protein by
the enzyme-linked immune sorbent assay. The surface plasmon resonance
assay further demonstrates that peptide no. 1 can specifically bind
to SARS-CoV-2 RBD with a binding affinity constant (Kd) of 5.8 μM. Transmission electron microscopy coupled
with a magnetic bead assay further confirms that the screened peptide
can specifically bind the inactivated SARS-CoV-2 virus. This SARS-CoV-2-specific
peptide holds great promise as a new bioreceptor/ligand for the rapid
and accurate detection of SARS-CoV-2.
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