mz9b00465_si_001.pdf (1.13 MB)
Peroxidase-Mediated In Situ Fabrication of Multi-Stimuli-Responsive and Dynamic Protein Nanogels from Tyrosine-Conjugated Biodynamer and Ovablumin
journal contribution
posted on 2019-09-13, 17:36 authored by Lin Wang, Li Liu, Bingyang Dong, Hanying ZhaoHorseradish
peroxidase (HRP)-mediated oxidation of tyrosine-conjugated
biodynamer containing acylhydrazone linkages and ovalbumin (OVA)/reduced
ovalbumin (rOVA) generated protein nanogels through simultaneous tyrosine
coupling and thiol cross-linking in the presence of H2O2. The obtained nanogels are multi-stimuli-responsive to temperature,
pH, and glutathione (GSH) and possess the dynamic character of reversible
covalent bonds. The OVA-based or rOVA-based protein nanogels can be
utilized as cargoes of anticancer drug curcumin (Cur). The Cur-loaded
protein nanogels rapidly released Cur in intracellular-mimicking acidic
and reductive environment, thus, making these protein nanogels promising
nanocarriers for intracellular drug delivery. HRP-mediated cross-linking
of the tyrosine-conjugated biodynamer and proteins provides a facile
and versatile approach for fabrication of responsive and adaptive
biohybrid nanogels under mild conditions.