American Chemical Society
ic2017648_si_001.pdf (1.32 MB)

Palladium(II) and Platinum(II) Bind Strongly to an Engineered Blue Copper Protein

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journal contribution
posted on 2011-11-21, 00:00 authored by Matthew P. McLaughlin, Thomas H. Darrah, Patrick L. Holland
Studies of palladium­(II) and platinum­(II) binding to well-characterized proteins contribute to understanding the influence of these metals in the environment and body. The well-characterized apoprotein of azurin has a soft-metal binding site that may be exposed to solvent by mutation of a coordinating His-117 residue to glycine (H117G). Palladium­(II) and platinum­(II) form strong 1:1 adducts with the apo form of H117G azurin. A combination of UV–vis, circular dichroism, and inductively coupled plasma mass spectrometry techniques suggests that the metal binds specifically at His-46 and Cys-112 of the protein.