Paclitaxel Is an Inhibitor and Its Boron Dipyrromethene Derivative Is a Fluorescent Recognition Agent for Botulinum Neurotoxin Subtype A
journal contributionposted on 11.04.2013, 00:00 by Saedeh Dadgar, Zack Ramjan, Wely B. Floriano
We have successfully identified one new inhibitor and one new fluorescent recognition agent for the botulinum neurotoxin subtype A (BoNT/A) using the virtual screening protocol “protein scanning with virtual ligand screening” (PSVLS). Hit selection used an in-house developed holistic binding scoring method. Selected hits were tested experimentally for inhibitory activity using fluorescence resonance energy transfer (FRET) assays against the light chain (catalytic domain) of BoNT/A. Ligand binding was determined against the light and heavy chain BoNT/A complex through either radiolabeled ligand binding assays (nonfluorescent ligands) or fluorescence intensity assays (fluorescent ligands). These experimental assays have confirmed one compound (paclitaxel) to inhibit BoNT/A’s proteolytic activity experimentally with an IC50 of 5.2 μM. A fluorescent derivative was also confirmed to bind to the toxin and therefore is a suitable candidate for the rational design of new detection agents and for the development of fluorescence-based multiprobe detection assays.
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fluorescence resonance energy transferrecognition agentdetection agentsPSVLSnonfluorescent ligandsSelected hitsscreeningbotulinum neurotoxin subtypeFluorescent Recognition Agentlight chainBoNTfluorescence intensity assaysIC 50FRET5.2 μ MBotulinum Neurotoxin Subtype AWeradiolabeled ligand binding assaysBoron Dipyrromethene