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Oxazolidinone-Mediated Sequence Determination of One-Bead One-Compound Cyclic Peptide Libraries

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journal contribution
posted on 04.04.2018, 17:48 authored by Hader E. Elashal, Ryan D. Cohen, Heidi E. Elashal, Monika Raj
A novel one-bead one-compound (OBOC) dual ring-opening/cleavage approach for cyclic peptide sequencing was developed. The method selectively modifies serine, cysteine, threonine, and/or glutamic acid to an oxazolidinone-derived moiety, thereby increasing the susceptibility of the modified peptide backbone toward hydrolysis. The resulting linear peptide was then sequenced in 1 min by tandem mass spectrometry on a quadrupole time-of-flight instrument incorporating two-dimensional liquid chromatography and ion mobility spectrometry separation. To evaluate this approach, a library of cyclic peptides was successfully sequenced with 98% overall accuracy, demonstrating its robustness and broad substrate scope.