jf3053862_si_001.pdf (268.64 kB)
Optimized GC-MS Method To Simultaneously Quantify Acetylated Aldose, Ketose, and Alditol for Plant Tissues Based on Derivatization in a Methyl Sulfoxide/1-Methylimidazole System
journal contribution
posted on 2016-02-19, 10:35 authored by Ke Li, Shichang Liu, Yupeng Tan, Nan Chao, Xiaoming Tian, Liwang Qi, William A. Powell, Xiangning Jiang, Ying GaiThe isomers of monosaccharide always
produce multiple chromatographic
peaks as volatile derivatives during gas chromatography, which may
result in the overlapping of different sugar peaks. Whereas reduction
and oximation of sugar carbonyl groups for GC analysis do eliminate
many isomer derivatives, the approaches create new problems. One ketose
can yield two peaks by oximation, and different aldoses and ketoses
can yield the same alditol upon reduction, leading to the inability
to detect some important monosaccharides. This paper reports an optimal
method that yields a single peak per sugar by acetylation directly.
By using a methyl sulfoxide (Me2SO)/1-methylimidazole (1-MeIm)
system, the carbohydrates in acetic anhydride (Ac2O) esterification
reactions were solubilized, and the oxidation that normally occurs
was inhibited. The results demonstrate that acetylated derivatives
of 23 saccharides had unique peaks, which indicates aldose, ketose,
and alditol can be determined simultaneously by GC-MS.