ac401609c_si_001.pdf (1.76 MB)
Optically Optimized Photoluminescent and Interferometric Biosensors Based on Nanoporous Anodic Alumina: A Comparison
journal contribution
posted on 2013-08-20, 00:00 authored by Abel Santos, Tushar Kumeria, Dusan LosicHerein,
we present a comparative study about the sensing performance
of optical biosensors based on photoluminescence spectroscopy (PLS)
and reflectometric interference spectroscopy (RIfS) combined with
nanoporous anodic alumina (NAA) platforms when detecting different
analytes under distinct adsorption conditions. First, NAA platforms
are structurally engineered in order for optimizing the optical signals
obtained by PLS and RIfS. Then, the most optimal NAA platforms combined
with PLS and RIfS are quantitatively compared by detecting two different
analytes: d-glucose and l-cysteine under nonspecific
and specific adsorption conditions, respectively. The obtained results
demonstrate that such parameters as the analyte nature and adsorption
conditions play a direct role in the sensing performance of these
platforms. However, as this study demonstrates, PLS-NAA platforms
are more sensitive than RIfS-NAA ones. The former shows better linearity
(i.e., proportional change in the sensing parameter with analyte concentration),
higher sensitivity toward analytes (i.e., sharper change in the sensing
parameter with analyte concentration), and lower limit of detection
(i.e., minimum detectable concentration of analyte).