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One-Pot Generating Subunit Vaccine with High Encapsulating Efficiency and Fast Lysosome Escape for Potent Cellular Immune Response
journal contribution
posted on 2020-07-17, 22:03 authored by Pengyan Hao, Xiaoxiao Wu, Liwen Wang, Shaoyin Wei, Hongyan Xu, Wenjuan Huang, Yana Li, Tinghong Zhang, Xingjie ZanUtilizing
nanoparticles to deliver subunit vaccine is considered
to be a promising strategy to improve immune response. However, currently
reported systems suffered from one or more points, for example, delicate
design on molecular structures and elaborate synthesis process, low
antigen and/or adjuvant encapsulation efficiency, involvement of toxic
materials, and denaturing of bioactivity of antigen and/or adjuvant.
To address these issues, here, for the first time, we developed a
one-pot method to produce a subunit vaccine by using hexa-histidine
metal assembly (HmA) to codeliver tumor-associated antigens (GP100,
a peptide KTWGQYWQV) and adjuvant (CpG). The generation of subunit
vaccines was detailedly characterized by various techniques, including
dynamic scatter, scanning electron microscopy, transmission electron
microscopy, UV–visible spectroscopy, agarose gel electrophoresis,
etc. HmA displayed high efficiency on encapsulating both subunits
(GP100 and CpG) under mild conditions, and the generated subunit vaccine
showed a pH-dependent release profile of loaded subunits. In the cellular
tests, these subunit vaccines behaved with a quick endocytosis into
immune cells and a fast endo/lysosomes escape, inducing maturation
of antigen presentative cells and stimulating a potent cellular immune
response. These results suggested that HmA is a robust platform for
fabricating subunit vaccine, with immense potential for the immunotherapy
of various diseases.
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hexa-histidine metal assemblyGPFast Lysosome Escapeagarose gel electrophoresissubunit vaccinesUVPotent Cellular Immune Response Uti...One-Pot Generating Subunit VaccineHmAscanning electron microscopytransmission electron microscopycodeliver tumor-associated antigensKTWGQYWQVantigen presentative cellspH-dependent release profileadjuvantsubunit vaccineHigh Encapsulating Efficiency
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