posted on 2020-08-10, 04:46authored byFaith
Pwaniyibo Samson, Ambrose Teru Patrick, Tosin Esther Fabunmi, Muhammad Falalu Yahaya, Joshua Madu, Weilue He, Srinivas R. Sripathi, Jennifer Tyndall, Hayatu Raji, Donghyun Jee, Diana R. Gutsaeva, Wan Jin Jahng
The
current study determined the natural angiogenic molecules using
an unbiased metabolomics approach. A chick chorioallantoic membrane
(CAM) model was used to examine pro- and antiangiogenic molecules,
followed by gas chromatography–mass spectrometry (GCMS) analysis.
Vessel formation was analyzed quantitatively using the angiogenic
index (p < 0.05). At embryonic day one, a white
streak or circle area was observed when vessel formation begins. GCMS
analysis and database search demonstrated that angiogenesis may initiate
when oleic, cholesterol, and linoleic acids increased in the area
of angiogenic reactions. The gain of function study was conducted
by the injection of cholesterol and oleic acid into a chick embryo
to determine the role of each lipid in angiogenesis. We propose that
oleic acid, cholesterol, and linoleic acid are natural molecules that
set the platform for the initiation stage of angiogenesis before other
proteins including the vascular endothelial growth factor, angiopoietin,
angiotensin, and erythropoietin join as the angiome in sprout extension
and vessel maturation.