posted on 2023-03-28, 16:34authored byHsiu-Chung Tu, Yun Tang, Yi-Hua Lee, Fang-Ming Chen, Po-Chiao Lin
Breast cancer (BC) is one of the most common malignancies
in the
world with aberrantly expressed glycans. The different types and stages
still limit a comprehensive method in the prediagnosis of BC patients.
In this research, a synthetic boronic acid-disulfide (BASS) probe
has been developed for the two steps of O → S → N acyl
transfer in glycoprotein recognition and labeling. The specificity
and sensitivity of this method have been carefully studied in the
case of immunoglobulin G, and the labeling efficiency was determined
up to 60%. The BASS-functionalized slide is a powerful platform for
monitoring the alteration of glycan patterns in human sera. Compared
to the samples from healthy individuals, sera of BC patients gave
specific patterns to eight lectins binding. The BASS-directed glycoprotein
strategy promises a rapid sensing platform for a high-throughput screening
of clinical BC samples and could be easily applied to other cancer
prediagnoses.