ja503215w_si_001.pdf (4.33 MB)
Nucleic Acid–Metal Organic Framework (MOF) Nanoparticle Conjugates
journal contribution
posted on 2014-05-21, 00:00 authored by William Morris, William
E. Briley, Evelyn Auyeung, Maria D. Cabezas, Chad A. MirkinNanoparticles of
a metal–organic framework (MOF), UiO-66-N3 (Zr6O4OH4(C8H3O4–N3)6), were synthesized.
The surface of the MOF was covalently functionalized with oligonucleotides,
utilizing a strain promoted click reaction between DNA appended with
dibenzylcyclooctyne and azide-functionalized UiO-66-N3 to create the first MOF nanoparticle–nucleic acid
conjugates. The structure of the framework was preserved throughout
the chemical transformation, and the surface coverage of DNA was quantified.
Due to the small pore sizes, the particles are only modified on their
surfaces. When dispersed in aqueous NaCl, they exhibit increased stability
and enhanced cellular uptake when compared with unfunctionalized MOF
particles of comparable size.