posted on 2021-03-24, 05:13authored byShiqi Huang, Limin Zhang, Liyi Dai, Yuanyuan Wang, Yang Tian
There is still an urgent need to
develop reliable analytical methods
of O2•– in vivo for deeply elucidating
the roles of O2•– playing in the
brain. Herein, a nonenzymatic electrochemical sensor with ratiometric
signal output was developed for an in vivo analysis of O2•– in the rat brain. Diphenylphosphonate-2-naphthol
ester (ND) was designed and synthesized as a specific recognition
molecule for the selective determination of O2•–. An anodic peak ascribed to the oxidation of 2-naphthol was generated
via the nucleophilic substitution between ND and O2•– and was increased with the increasing concentration
of O2•–. Meanwhile, the inner
reference of methylene blue (MB) was co-assembled at the electrode
surface to enhance the determination accuracy of O2•–. The anodic peak current ratio between 2-naphthol
and MB exhibited a good linear relationship with the concentration
of O2•– from 2 to 200 μM.
Because of the stable molecule character of ND and its specific reaction
with O2•–, the developed electrochemical
sensor demonstrated excellent selectivity toward various potential
interferences in the brain and good stability even after storage for
7 days. Accordingly, the present electrochemical sensor with high
selectivity, high stability, and high accuracy was successfully exploited
in monitoring the levels of O2•– in the rat brain and that of the diabetic model followed by cerebral
ischemia.