posted on 2017-06-13, 00:00authored bySeong
Jun Park, Sanggeun Song, In-Chun Jeong, Hye Ran Koh, Ji-Hyun Kim, Jaeyoung Sung
Enzyme-to-enzyme
variation in the catalytic rate is ubiquitous
among single enzymes created from the same genetic information, which
persists over the lifetimes of living cells. Despite advances in single-enzyme
technologies, the lack of an enzyme reaction model accounting for
the heterogeneous activity of single enzymes has hindered a quantitative
understanding of the nonclassical stochastic outcome of single enzyme
systems. Here we present a new statistical kinetics and exactly solvable
models for clonal yet heterogeneous enzymes with possibly nonergodic
state dynamics and state-dependent reactivity, which enable a quantitative
understanding of modern single-enzyme experimental results for the
mean and fluctuation in the number of product molecules created by
single enzymes. We also propose a new experimental measure of the
heterogeneity and nonergodicity for a system of enzymes.