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Nitrilotriacetic Acid-Derivatized Quantum Dots for Simple Purification and Site-Selective Fluorescent Labeling of Active Proteins in a Single Step

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journal contribution
posted on 15.10.2008, 00:00 by Manish Gupta, Anne Caniard, Ángeles Touceda-Varela, Dominic J. Campopiano, Juan C. Mareque-Rivas
We demonstrate that QDs coated with nitrilotriacetic acid (NTA) bound to Ni2+ can be used to reversibly and selectively bind, purify, and fluorescently label His6-tagged (N-terminal) glutathione S-transferase (GST) in one step with retention of enzymatic activity. We find binding to be less effective in the absence of the His6-tag or Ni2+ ions.