The
microneedle (MN) provides a promising strategy for transdermal
delivery of exosomes (EXO), in which the therapeutic effects and clinical
applications are greatly reduced by the fact that EXO can only partially
reach the injury site by passive diffusion. Here, we designed a detachable
MN array to deliver EXO modified by a nitric oxide nanomotor (EXO/MBA)
for Achilles tendinopathy (AT) healing. With the releasing of EXO/MBA, l-arginine was converted to nitric oxide by NOS or ROS as the
driving force. Benefiting from the motion ability and the property
of MPC tending to lower pH, EXO could accumulate at the injury site
more efficiently. This work demonstrated that EXO/MBA-loaded MN notably
suppressed the inflammation of AT, facilitated the proliferation of
tendon cells, increased the expression of Col1a, and prevented extracellular
matrix degradation, indicating its potential value in enthesiopathy
healing and other related biomedical fields.