jf2c00823_si_001.pdf (15.48 MB)
New Lanostane-Type Triterpenes with Anti-Inflammatory Activity from the Epidermis of Wolfiporia cocos
journal contributionposted on 2022-03-29, 11:36 authored by Te-Ri-Gen Bao, Guo-Qing Long, Yong Wang, Qian Wang, Xuan-Li Liu, Gao-Sheng Hu, Xiao-Xu Gao, An-Hua Wang, Jing-Ming Jia
A chemical study on the epidermis of cultivated edible mushroom Wolfiporia cocos resulted in the isolation and identification of 46 lanostane triterpenoids, containing 17 new compounds (1–17). An experimental determination of their anti-inflammatory activity showed that poricoic acid GM (39) most strongly inhibited NO production in LPS-induced RAW264.7 murine macrophages with an IC50 value at 9.73 μM. Furthermore, poricoic acid GM induced HO-1 protein expression and inhibited iNOS and COX2 protein expression as well as the release of PGE2, IL-1β, IL-6, TNF-α, and reactive oxygen species (ROS) in LPS-induced RAW264.7 cells. Mechanistically, poricoic acid GM suppressed the phosphorylation of the IκBα protein, which prevented NF-κB from entering the nucleus to lose transcriptional activity and inhibited the dissociation of Keap1 from Nrf2, thereby activating Nrf2 into the nucleus to regulate antioxidant genes. Furthermore, the MAPK signaling pathway may play a significant role in poricoic acid GM-induced elimination of inflammation. This work further confirms that lanostane triterpenoids are key ingredients responsible for the anti-inflammatory properties of the edible medicinal mushroom W. cocos.
regulate antioxidant genesreactive oxygen speciesporicoic acid gmkey ingredients responsiblelose transcriptional activitycox2 protein expression1 protein expression7 murine macrophagesthereby activating nrf2inflammatory activity showed50 sub46 lanostane triterpenoidsinflammatory activitylanostane triterpenoidsiκbα protein7 cellsnew lanostaneinflammatory propertiestype triterpenessignificant roleprevented nfinduced raw264induced eliminationexperimental determinationchemical study73 μm