posted on 2024-01-31, 16:42authored byDongdong Wang, Wei Jiang, Mehal Churiwal, Katrina Jia, Sarath P. D. Senadeera, Heidi R. Bokesch, Girma M. Woldemichael, Yong Kim, Robert G. Hawley, Jun S. Wei, Javed Khan, Barry R. O’Keefe, John A. Beutler, Kirk R. Gustafson
Neopetrotaurines A–C (1–3), unusual alkaloids possessing two
isoquinoline-derived moieties
that are linked via a unique taurine bridge, were isolated from a Neopetrosia sp. marine sponge. These new compounds have
proton-deficient structural scaffolds that are difficult to unambiguously
assign using only conventional 2- and 3-bond 1H–13C and 1H–15N heteronuclear correlation
data. Thus, the application of LR-HSQMBC and HMBC NMR experiments
optimized to detect 4- and 5-bond long-range 1H–13C heteronuclear correlations facilitated the structure elucidation
of these unusual taurine-bridged marine metabolites. Neopetrotaurines
A–C (1–3) showed significant
inhibition of transcription driven by the oncogenic fusion protein
PAX3-FOXO1, which is associated with alveolar rhabdomyosarcoma, and
cytotoxic activity against PAX3-FOXO1-positive cell lines.