Near-Infrared Quantum Dot and 89Zr Dual-Labeled Nanoparticles for in Vivo Cerenkov Imaging
journal contributionposted on 2016-12-27, 00:00 authored by Yiming Zhao, Travis M. Shaffer, Sudeep Das, Carlos Pérez-Medina, Willem J. M. Mulder, Jan Grimm
Cerenkov luminescence (CL) is an emerging imaging modality that utilizes the light generated during the radioactive decay of many clinical used isotopes. Although it is increasingly used for background-free imaging and deep tissue photodynamic therapy, in vivo applications of CL suffer from limited tissue penetration. Here, we propose to use quantum dots (QDs) as spectral converters that can transfer the CL UV-blue emissions to near-infrared light that is less scattered or absorbed in vivo. Experiments on tissue phantoms showed enhanced penetration depth and increased transmitted intensity for CL in the presence of near-infrared (NIR) QDs. To realize this concept for in vivo imaging applications, we developed three types of NIR QDs and 89Zr dual-labeled nanoparticles based on lipid micelles, nanoemulsions, and polymeric nanoplatforms, which enable codelivery of the radionuclide and the QDs for maximized spectral conversion efficiency. We finally demonstrated the application of these self-illuminating nanoparticles for imaging of lymph nodes and tumors in a prostate cancer mouse model.
background-free imagingprostate cancer mouse modelNear-Infrared Quantum Dotuse quantum dotsnear-infrared lightvivo imaging applicationslymph nodeslipid micellesself-illuminating nanoparticles89 Zr dual-labeled nanoparticlestissue phantomsvivo applicationstissue photodynamic therapyVivo Cerenkov Imaging Cerenkov luminescenceimaging modalityconversion efficiency89 Zr Dual-Labeled NanoparticlesNIR QDspenetration depthCL UV-blue emissionstissue penetration