American Chemical Society
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Near-Infrared Perylenecarboximide Fluorophores for Live-Cell Super-Resolution Imaging

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journal contribution
posted on 2024-03-05, 16:37 authored by Ze-Hua Wu, Xingfu Zhu, Qiqi Yang, Yulian Zagranyarski, Krishna Mishra, Hilmar Strickfaden, Ronald P. Wong, Thomas Basché, Kaloian Koynov, Mischa Bonn, Chen Li, Xiaomin Liu, Klaus Müllen
Organic near-infrared (NIR) photoblinking fluorophores are highly desirable for live-cell super-resolution imaging based on single-molecule localization microscopy (SMLM). Herein we introduce a novel small chromophore, PMIP, through the fusion of perylenecarboximide with 2,2-dimetheylpyrimidine. PMIP exhibits an emission maximum at 732 nm with a high fluorescence quantum yield of 60% in the wavelength range of 700–1000 nm and excellent photoblinking without any additives. With resorcinol-functionalized PMIP (PMIP-OH), NIR SMLM imaging of lysosomes is demonstrated for the first time in living mammalian cells under physiological conditions. Moreover, metabolically labeled nascent DNA is site-specifically detected using azido-functionalized PMIP (PMIP-N3) via click chemistry, thereby enabling the super-resolution imaging of nascent DNA in phosphate-buffered saline with a 9-fold improvement in spatial resolution. These results indicate the potential of PMIP-based NIR blinking fluorophores for biological applications of SMLM.