am0c11635_si_001.pdf (1.63 MB)
Near-Infrared-Induced Contractile Proteinosome Microreactor with a Fast Control on Enzymatic Reactions
journal contribution
posted on 2020-09-02, 20:43 authored by Jiaojiao Su, Haixu Chen, Zhijun Xu, Shengliang Wang, Xiaoman Liu, Lei Wang, Xin HuangInspired
by the compartmentalized structure of cells, self-regulating
responsive hollow microcapsules are highly desirable for the modulation
of enzymatic reactions. Here, we report a strategy to fabricate gold
nanorod embedded proteinosomes by covalently grafting gold nanorods
onto the surface of proteinosomes. The excellent photothermal conversion
efficiency of the embedded gold nanorod and the thermal phase transition
of the grafted PNIPAAm allow the constructed hybrid proteinosomes
to show reversible contraction behaviors triggered by near-infrared
light with the molecular weight cutoff of the membrane decreased to
ca. 50 kDa, and importantly, the contraction frequency of the constructed
proteinosomes could be as fast as 1 min and last for at least 15 cycles.
Subsequently, the effective encapsulation of three cascade enzymes
into the proteinosomes realizes the construction of a near-infrared
responsive microreactor that allows control of the cascade reaction
by near-infrared illumination, thereby enabling reversible on and off of the enzymatic reaction. Such microcapsule-based reactors
demonstrate the potential to alter the membrane molecular weight cutoff,
and it is believed that the development of such responsive microcapsules
will have great potential for studying cellular responses and provide
a platform for future applications in biosensing and drug delivery.