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Download fileNatural Salep/PEGylated Chitosan Double Layer toward a More Sustainable pH-Responsive Magnetite Nanocarrier for Targeted Delivery of DOX and Hyperthermia Application
journal contribution
posted on 2019-01-11, 00:00 authored by Nasrin Zohreh, Sakineh Alipour, Seyed Hassan Hosseini, Morena S. Xaba, Reinout Meijboom, Mahdi Fasihi Ramandi, Nazila Gholipour, Mehdi AkhlaghiTwo pH-responsive
polysaccharide-based magnetite nanocarriers have
been developed from modified salep and PEGylated chitosan decorated
onto the surface of magnetite nanoparticles for active loading and
targeted delivery of doxorubicin. The first nanocarrier was formed
via imine bond formation and electrostatic hydrogen bonding interactions
between dialdehyde salep-modified magnetite nanoparticles and DOX
in a one-layered nanocarrier fashion. Subsequently, PEGylated chitosan
was used as a second layer on the surface of the first nanocarrier,
mainly by the same interactions, producing a double-layered polysaccharide-based
nanocarrier. In vitro release studies indicated that
the use of PEGylated chitosan as the second shell provides more control
on the rate and amount of DOX release and makes double-layered nanocarrier
more pH-sensitive. Magnetic heating capacity of double-layered nanocarrier
was investigated and release profile under AMF (42 °C, magnetically
induced) showed a good improvement in the time and amount of DOX release. In vitro MTT assays depicted that the DOX-loaded double-layered
nanocarrier produces a large cytotoxic response to HeLa cells, which
is comparable with free DOX in higher concentration. DOX-free double-layered
nanocarrier also exhibited low cytotoxicity against normal cells,
an indication of their excellent biocompatibility. Application of
AMF also showed a large effect on the cytotoxicity of the nanocarrier
rather than without AMF condition. Investigation on cellular uptake
of nanocarrier revealed the high targeting ability of nanocarrier
toward HeLa cells, especially in the presence of AMF. Blood compatibility
investigations indicated that hemolysis of RBCs and coagulation times
fall into the normal range for blood in the presence of DOX-free and
DOX-loaded double-layered nanocarrier.
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Keywords
one-layered nanocarrier fashiondouble-layered polysaccharide-based nanocarrierPEGylated chitosanDOX-free double-layered nanocarriercoagulation times fallAMFMagnetic heating capacityHeLa cellsSustainable pH-Responsive Magnetite Nanocarrierdouble-layered nanocarrierDOX releaseDOX-loaded double-layered nanocarrierblood compatibility investigationsMTTimine bond formationdialdehyde salep-modified magnetite nanoparticlesRBCpH-responsive polysaccharide-based magnetite nanocarriers