posted on 2021-04-08, 12:04authored bySara Borrego-González, Berta de la Cerda, Francisco J. Díaz-Corrales, Aránzazu Díaz-Cuenca
Human
induced pluripotent stem cells (hiPSCs) represent the most
promising biological material for regenerative medicine applications.
In this work, a 3D solid nanofibrous matrix of defined composition
(Colamigel-S) consisting of 97 wt % gelatin, 2.6 wt % atelocollagen,
and 0.4 wt % laminin has been reproducibly processed and characterized
and exhibits a homogeneous nanofibrillar network of high surface area,
interconnected microcavities, and typical D-periodic collagen fibril
nanostructural features. The purpose of the study was to test the
performance of Colamigel-S as substrate for in vitro hiPSCs culture,
finding that these cells efficiently attach and grow keeping their
characteristic stem morphology and undifferentiated state.