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Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
journal contribution
posted on 2017-09-08, 00:00 authored by Xiang Ren, Junrong Yan, Dan Wu, Qin Wei, Yakun WanAlzheimer’s disease (AD) biomarkers
can reflect the neurochemical
indicators used to estimate the risk in clinical nephrology. Apolipoprotein
E (ApoE) is an early biomarker for AD in clinical diagnosis. In this
research, through bactrian camel immunization, lymphocyte isolation,
RNA extraction, and library construction, ApoE-specific Nbs with high
affinity were successfully separated from an immune phage display
nanobody library. Herein, a colorimetric immunosensor was developed
for the point-of-care testing of ApoE by layer-by-layer nanoassembly
techniques and novel nanobodies (Nbs). Using highly oriented Nbs as
the capture and detection antibodies, an on-site immunosensor was
developed by detecting the mean gray value of fade color due to the
glutaraldehyde@3-aminopropyltrimethoxysilane oxidation by H2O2. The detection limit of AopE is 0.42 pg/mL, and the
clinical analysis achieves a good performance. The novel easily operated
immunosensor may have potential application in the clinical diagnosis
and real-time monitoring for AD.
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RNA extractionApolipoprotein Ecolorimetric immunosensorneurochemical indicatorsbiomarkerdetection limitADbactrian camel immunizationApoE-specific Nbsdetection antibodieslayer-by-layer nanoassembly techniquesNanobody-Based Apolipoprotein E ImmunosensorH 2 O 2lymphocyte isolationlibrary constructionpoint-of-care testingdiagnosisnovel nanobodiesphage display nanobody library
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