posted on 2023-09-06, 17:47authored byLiangqin Liu, Jun Deng, Yinlong Wang, Xin He, Huibing He, Xiaopeng Chen, Dankui Liao, Zhangfa Tong
Nanozymes, enzyme-mimicking nanomaterials, have attracted
increasing
attention due to their low cost, high stability, and catalytic ability
compared with natural enzymes. However, the catalytic efficiency of
the nanozymes is still relatively low, and catalytic reaction mechanisms
remain unclear. To address these issues, herein we prepared nitrogen-riched
and sulfur-codoped nano hollow carbons (N/S-HCS) using a green and
useful template of CaCO3. N/S-HCS exhibits enhanced oxidase-like
activity and catalytic kinetic performance. It could directly oxidize
the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to
the heavy blue colored ox-TMB without H2O2.
The maximum reaction rate (Vmax) is 186.7
× 10–8 M·s–1, and Michaelis–Menten
constant (Km) is 0.162 mM. DFT results
show that N and S codoping could work synergistically to provide more
active sites, resulting in the superior ability to adsorb oxygen and
enhanced catalytic activity. Meantime, we develop a multispectral
characterization strategy to unravel catalytic reaction mechanisms
about N/S-HCS. It successfully induces the generation of superoxide
(•O2–) and hydroxyl
(•OH) during the colorimetric reaction which are
the key intermediate products of the catalytic reaction. Furthermore,
N/S-HCS increased the cellular reactive oxygen species level significantly
and induced bacteriostasis to more than 95% of Escherichia
coli.