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NMR Spectroscopic Determination of Angles α and ζ in RNA from CH-Dipolar Coupling, P-CSA Cross-Correlated Relaxation

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journal contribution
posted on 06.12.2000, 00:00 by Christian Richter, Bernd Reif, Christian Griesinger, Harald Schwalbe
A new method is introduced to measure the backbone torsion angles α and ζ in 13C-labeled oligonucleotides. The experiments relies on the quantification of the cross-correlated relaxation of C,P double and zero quantum coherence caused by the C,H dipolar coupling and the P chemical shift anisotropy. Two-dimensional surfaces that reveal the angular dependence of the cross-correlated relaxation rates depend on the backbone angles α and β as well as ε and ζ and are interpreted using torsion angle information for the angles β and ε from experiments measuring 3J(H,P) and 3J(C,P) coupling constants. The experiments have been carried out on the 10mer RNA 5‘-CGCUUUUGCG-3‘ that forms a hairpin and in which the four uridine residues are 13C-labeled in the ribofuranoside moiety.

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