posted on 2005-09-14, 00:00authored byYi-Ping Ho, Matthew C. Kung, Samuel Yang, Tza-Huei Wang
We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence
of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation
of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual
nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using
this novel biosensing method as proof-of-concept.