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Multiplexed Detection of Epigenetic Markers Using Quantum Dot (QD)-Encoded Hydrogel Microparticles
journal contribution
posted on 2016-03-14, 00:00 authored by Sang Yun Yeom, Choong
Hyun Son, Byung Sun Kim, Sung Hyun Tag, Eunjoo Nam, Hyogeun Shin, So Hyun Kim, Haemin Gang, Hyunjoo
J. Lee, Jungkyu Choi, Heh-In Im, Il-Joo Cho, Nakwon ChoiEpigenetic
alterations in gene expression are influenced by experiences
and environment, resulting in significant variation of epigenetic
markers from individual to individual. Therefore, it is imperative
to measure various epigenetic markers simultaneously from samples
of individual subjects to accurately analyze the epigenetic markers
in biological samples. Moreover, the individualized genome-wide analysis
has become a critical technology for recent trends in clinical applications
such as early diagnosis and personalized medicine screening of numerous
diseases. The array-based detection of modified histones, conventionally
used for multiplexed analysis of epigenetic changes, requires pooling
of samples from many subjects to analyze population-wise differences
in the expression of histone markers and does not permit individualized
analysis. Here, we report multiplexed detection of genome-wide changes
in various histone modifications at a single-residue resolution using
quantum dot (QD)-encoded polyethylene glycol diacrylate (PEGDA) hydrogel
microparticles. To demonstrate the potential of our methodology, we
present the simultaneous detection of (1) acetylation of lysine 9
of histone 3 (Ac–H3K9), (2) dimethylation of H3K9 (2Me-H3K9),
and (3) trimethylation of H3K9 (3Me-H3K9) from three distinct regions
in the brain [nucleus accumbens (NAc), dorsal striatum (DSt), and
cerebellum (Cbl)] of cocaine-exposed mice. Our hydrogel-based epigenetic
assay enabled relative quantification of the three histone variants
from only 10 μL of each brain lysate (protein content = ∼
1 μg/μL) per mouse. We verified that the exposure to cocaine
induced a significant increase of acetylation while a notable decrease
in methylation in NAc.
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medicine screeningNAchistone variantsreport multiplexed detectionbrain lysatehydrogel microparticlesEpigenetic Markershistone modificationsPEGDAhistone markers3Khistone 3subjectgene expressionnucleus accumbensQuantum Dotquantum dot10 μ Llysine 9acetylationprotein contentsamplemultiplexed analysisMultiplexed DetectionQD
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