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Download fileMultiphoton Activation of Photo-Strain-Promoted Azide Alkyne Cycloaddition “Click” Reagents Enables in Situ Labeling with Submicrometer Resolution
journal contribution
posted on 2017-09-19, 00:00 authored by Christopher
D. McNitt, Hazel Cheng, Susanne Ullrich, Vladimir V. Popik, Matthew BjerknesIrradiation
of cyclopropenone-masked dibenzocyclooctynes with near-infrared
pulses from a femtosecond laser triggers photodecarbonylation via
nonresonant two- or three-photon excitation. Multiphoton-generated
cyclooctynes undergo a SPAAC reaction with organic azides, yielding
the expected triazoles. Multiphoton-triggered SPAAC (MP-SPAAC) enables
high resolution 3-D photoclick derivatization of hydrogels and tissues.
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femtosecond laser triggers photodecarbonylationAzidetissuehydrogelthree-photon excitationEnableMultiphoton-triggered SPAACazideReagentCycloadditionMP-SPAACnear-infrared pulsesSPAAC reactionresolution 3- D photoclick derivatizationcyclopropenone-masked dibenzocyclooctynesAlkynetriazoleMultiphoton-generated cyclooctynesSitunonresonantMultiphoton ActivationPhoto-Strain-PromotedSubmicrometer Resolution Irradiation
