posted on 2017-03-14, 16:35authored byWei Qi, Jing Yan, Haifeng Sun, Hua Wang
The development of novel materials
capable of delivering multiple
growth factors is urgent and essential for rapid and effective tissue
regeneration. In this study, a kind of composite film composed of
poly-l-lysine (PLL), heparin (Hep), and Au nanoparitcles
(Au nps) has been fabricated to deliver the basic fibroblast growth
factor (bFGF) and bone morphogenetic protein-2 (BMP-2) simultaneously.
The films have been found to show enhanced mechanical property due
to the incorporation of Au nps. They have also shown good anticoagulation
activity with long activated partial thromboplastin time because of
the contribution of Hep molecules. Moreover, the osteogenesis studies
reveal that the loaded bFGF and BMP-2 in the composite films have
a synergistic differentiation effect on mesenchymal stem cells, as
indicated by alkaline phosphatase (ALP) activity assay and collagen
type I (Col-I) gene expression. In contrast to the (PLL/Hep)6/BMP-2/(PLL/Au nps)6/(PLL/Hep)6 and (PLL/Hep)6/(PLL/Au nps)6/(PLL/Hep)6/bFGF films,
the (PLL/Hep)6/BMP-2/(PLL/Au nps)6/(PLL/Hep)6/bFGF films have shown higher ALP activity and higher Col-I
expression level. Therefore, the developed multifunctional films could
be potentially used as osteoinductive coatings of biomaterials. Particularly,
this simple and convenient strategy provides an effective approach
for the immobilization of multiple growth factors, which may be extended
to other bioactive systems for the development of novel multifunctional
bioactive surfaces.