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Download fileMonitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles
journal contribution
posted on 2018-02-05, 00:00 authored by Ashley
E. Augspurger, Xiaoxing Sun, Brian G. Trewyn, Ning Fang, Anthony S. StenderTo
establish a new method for tracking the interaction of nanoparticles
with chemical cleaving agents, we exploited the optical effects caused
by attaching 5–10 nm gold nanoparticles with molecular linkers
to large mesoporous silica nanoparticles (MSN). At low levels of gold
loading onto MSN, the optical spectra resemble colloidal suspensions
of gold. As the gold is removed, by cleaving agents, the MSN revert
to the optical spectra typical of bare silica. Time-lapse images of
gold-capped MSN stationed in microchannels reveal that the rate of
gold release is dependent on the concentration of the cleaving agent.
The uncapping process was also monitored successfully for MSN endocytosed
by A549 cancer cells, which produce the cleaving agent glutathione.
These experiments demonstrate that the optical properties of MSN can
be used to directly monitor cleaving kinetics, even in complex cellular
settings.
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Keywords
549 cancer cellsTime-lapse imageschemical cleaving agentscleaving agentsmesoporous silica nanoparticlescleaving agent glutathionecleaving kineticsGold-Capped Mesoporous Silica NanoparticlesStimulated Uncapping Processcleaving agentgold loadinguncapping processMSN endocytosedgold releasegold-capped MSN