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Download fileMolecular Identification of Tumor-Derived Extracellular Vesicles Using Thermophoresis-Mediated DNA Computation
journal contribution
posted on 2021-01-18, 06:06 authored by Yike Li, Jinqi Deng, Ziwei Han, Chao Liu, Fei Tian, Rui Xu, Da Han, Shaohua Zhang, Jiashu SunMolecular
profiling of tumor-derived extracellular vesicles (tEVs)
holds great promise for non-invasive cancer diagnosis. However, sensitive
and accurate identification of tEVs is challenged by the heterogeneity
of EV phenotypes which reflect different cell origins. Here we present
a DNA computation device mediated by thermophoresis for detection
of tEVs. The strategy leverages the aptamer-based logic gate using
multiple protein biomarkers on single EVs as the input and thermophoretic
accumulation to amplify the output signals for highly sensitive and
specific profiling of tEVs. Employing this platform, we demonstrate
a high accuracy of 97% for discrimination of breast cancer (BC) patients
and healthy donors in a clinical cohort (n = 30).
Furthermore, molecular phenotyping assessed by tEVs is in concordance
with the results from tissue biopsy in BC patients. The thermophoresis-mediated
molecular computation on EVs thus provides new opportunities for accurate
detection and classification of cancers.
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strategy leveragesMolecular Identificationthermophoretic accumulationEV phenotypesBC patientscancer diagnosisdetectioncell originsTumor-Derived Extracellular Vesiclestissue biopsyoutput signalsprotein biomarkersaptamer-based logic gatebreast cancertumor-derived extracellular vesiclestEVDNA computation deviceThermophoresis-Mediated DNA Computa...