ac3c03194_si_001.pdf (1.16 MB)
Mn2+-Triggered Swing-Arm Robot Strategy Using Anemone-Like Thi@AuPd@Cu-MOFs as Signaling Probes for the Detection of T‑2 Toxin
journal contributionposted on 2023-12-19, 03:03 authored by Yidan Zhang, Baoshan He, Yuling Wang, Jinshui Wang, Ying Liang, Huali Jin, Min Wei, Wenjie Ren, Zhiguang Suo, Yiwei Xu
Herein, we synthesized anemone-like copper-based metal–organic frameworks (MOFs) loaded with gold–palladium nanoparticles (AuPd@Cu-MOFs) and polyethylenimine-reduced graphene oxide/gold–silver nanosheet composites (PEI-rGO/AuAg NSs) for the first time to construct the sensor and to detect T-2 toxin (T-2) using triple helix molecular switch (THMS) and signal amplification by swing-arm robot. The aptasensor used PEI-rGO/hexagonal AuAg NSs as the electrode modification materials and anemone-like AuPd@Cu-MOFs as the signal materials. The prepared PEI-rGO/hexagonal AuAg NSs had a large specific surface area, excellent electrical conductivity, and good stability, which successfully improved the electrochemical performance of the sensors. The AuPd@Cu-MOFs with high porosity provided a great deal of attachment sites for the signaling molecule thionine (Thi), thereby increasing the signal response. The aptasensor developed in this study demonstrated a remarkable detection limit of 0.054 fg mL–1 under optimized conditions. Furthermore, the successful detection of T-2 in real samples was achieved using the fabricated sensor. The simplicity of the THMS-based method, which entails modifying the aptamer sequence, allows for easy adaptation to different target analytes. Thus, the sensor holds immense potential for applications in quality supervision and food safety.
reduced graphene oxidehigh porosity providedexcellent electrical conductivitydifferent target analytes054 fg mlsignaling molecule thionineelectrode modification materialsremarkable detection limithexagonal auag nssaptasensor used peiauag nsssignaling probessignal materialsaptasensor developedsuccessfully improvedsuccessful detectionstudy demonstratedsignal responsesignal amplificationreal samplesquality supervisionprepared peioptimized conditionslike thilike coppergreat dealgood stabilityfood safetyfirst timeentails modifyingelectrochemical performanceeasy adaptationbased methodattachment sitesarm robotaptamer sequenceachieved using