MnO2 Nanospheres Assisted by Cysteine Combined
with MnO2 Nanosheets as a Fluorescence Resonance Energy
Transfer System for “Switch-on” Detection of Glutathione
posted on 2021-07-01, 17:12authored byYan Li, Libing Zhang, Zhen Zhang, Yu Liu, Jing Chen, Jia Liu, Peiyao Du, Huixia Guo, Xiaoquan Lu
Manganese
dioxide nanosheets combined with cysteine-assisted emitting
manganese dioxide nanospheres (Cys-MnO2 nanospheres) is
fabricated for the first time as an “off–on”
fluorescence detection platform for glutathione (GSH). In this sensing
system, Cys-MnO2 nanospheres served as energy donors, while
MnO2 nanosheets were used as both energy acceptors and
recognition units. MnO2 nanosheets can effectively quench
the fluorescence of Cys-MnO2 nanospheres through the fluorescence
resonance energy transfer (FRET). The addition of GSH could reduce
MnO2 nanosheets into Mn2+, disrupting the FRET
process and restoring the fluorescence of Cys-MnO2 nanospheres.
Under the optimum conditions, the “switch-on” platform
we established has a wide response to GSH with a range of 5–50
μM and 150–800 μM, as well as a superior specificity.
Importantly, all components of the sensor are nontoxic, biocompatible,
easily prepared, and have a high utilization of raw materials. Moreover,
the sensing system achieved satisfactory results in human serum, showing
a tremendous potential in the field of biomedicine.