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MiniBacillus PG10 as a Convenient and Effective Production Host for Lantibiotics
journal contribution
posted on 2020-06-30, 13:43 authored by Amanda
Y. van Tilburg, Auke J. van Heel, Jörg Stülke, Niels A. W. de Kok, Anne-Stéphanie Rueff, Oscar P. KuipersEfficient
bacterial cell factories are important for the screening
and characterization of potent antimicrobial peptides such as lantibiotics.
Although lantibiotic production systems have been established in Lactococcus lactis and Escherichia coli, the industrial workhorse Bacillus subtilis has
been left relatively unexplored as a lantibiotic production host.
Therefore, we tested different B. subtilis strains
for their ability to produce lantibiotic peptides by using the subtilin
modification and transport enzymes derived from the natural subtilin
producer B. subtilis ATCC 6633. Our study shows that
although B. subtilis ATCC 6633 and 168 are able to
produce various processed lantibiotic peptides, an evident advantage
of using either the 8-fold protease-deficient strain WB800 or the
genome-minimized B. subtilis 168 strain PG10 is the
lack of extracellular serine protease activity. Consequently, leader
processing of lantibiotic precursor peptides is circumvented and thus
potential toxicity toward the production host is prevented. Furthermore,
PG10 provides a clean secondary metabolic background and therefore
appears to be the most promising B. subtilis lantibiotic
production host. We demonstrate the production of various lantibiotic
precursor peptides by PG10 and show different options for their in vitro activation. Our study thus provides a convenient B. subtilis-based lantibiotic production system, which facilitates
the search for novel antimicrobial peptides.
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lantibiotic peptidessubtilis ATCC 6633Mini Bacillus PG 10extracellular serine protease activitysubtilis ATCC 6633.lantibiotic precursor peptidesprotease-deficient strain WB 800lantibiotic production systemssubtilis lantibiotic production hostnovel antimicrobial peptideslantibiotic production hostsubtilin producer BPG 10Effective Production Hostworkhorse Bacillus subtilislantibiotic production systemsubtilis 168 strain PG 10
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