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Microfluidic Particle Dam for Visual and Quantitative Detection of Lead Ions
journal contribution
posted on 2019-12-12, 18:06 authored by Gaobo Wang, Lok Ting Chu, Hogi Hartanto, William Budi Utomo, Reynard Aaron Pravasta, Ting-Hsuan ChenLead contamination in drinking water is a primary concern
in public
health, but it is difficult to monitor by end-users. Here, we provide
a rapid and power-free microfluidic particle dam which enables visual
quantification of lead ions (Pb2+) by the naked eye. GR-5
DNAzyme with extended termini can connect magnetic microparticles
(MMPs) and polystyrene microparticles (PMPs) by DNA hybridization,
forming “MMPs-GR-5-PMPs”. When Pb2+ is present,
GR-5 is cleaved, resulting in an increasing number of free PMPs. To
visually count the free PMPs, the solution is loaded to a capillary-driven
microfluidic device that consists of a magnetic separator to remove
the MMPs-GR-5-PMPs, followed by a particle dam that traps and accumulates
the free PMPs into a visual bar with growing length proportional to
the concentration of lead. The device achieved a limit of detection
at 2.12 nM (0.44 ppb), high selectivity (>20,000-fold) against
other
metal ions, high tolerance to different pH and water hardness, and
is compatible with tap water with a high recovery rate, enabling visual
quantification and user-friendly interface for rapid screening of
water safety.
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Keywords
DNA hybridizationquantificationpolystyrene microparticleswater safetypower-free microfluidic particle damcapillary-driven microfluidic devicemetal ionsQuantitative Detectionuser-friendly interfacerecovery ratedrinking waterPbwater hardnessMicrofluidic Particle DamMMPs-GR -5-PMPsparticle damLead Ions
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