posted on 2024-02-06, 06:03authored byYucong Zou, Xiaofeng Li, Xuan Xin, Haixia Xu, Guanglei Zhao
A novel yeast-mediated hydrogenation was developed for
the synthesis
of neohesperidin dihydrochalcone (NHDC) in high yields (over 83%).
Moreover, whole-cell catalytic hydrolysis was also designed to hydrolyze
NHDC into potential sweeteners, hesperetin dihydrochalcone-7-O-glucoside (HDC-G) and hesperetin dihydrochalcone (HDC).
The biohydrogenation was further combined with whole-cell hydrolysis
to achieve a one-pot two-step biosynthesis, utilizing yeast to hydrogenate
CC in the structure, while Aspergillus niger cells hydrolyze glycosides. The conversion of NHDC and the proportion
of hydrolysis products could be controlled by adjusting the catalysts,
the components of the reaction system, and the addition of glucose.
Furthermore, yeast-mediated biotransformation demonstrated superior
reaction stability and enhanced safety and employed more cost-effective
catalysts compared to the traditional chemical hydrogenation of NHDC
synthesis. This research not only provides a new route for NHDC production
but also offers a safe and flexible one-pot cascade biosynthetic platform
for the production of high-value compounds from citrus processing
wastes.