American Chemical Society
jp031188m_si_001.pdf (243.45 kB)

Micelles of Lipid−Oligonucleotide Conjugates:  Implications for Membrane Anchoring and Base Pairing

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journal contribution
posted on 2004-05-20, 00:00 authored by C. Gosse, A. Boutorine, I. Aujard, M. Chami, A. Kononov, E. Cogné-Laage, J.-F. Allemand, J. Li, L. Jullien
This report examines the organization properties of new fluorescent DNA-lipids, either alone in water or in interaction with 1-octyl-β-d-glucopyranoside micelles or egg phosphatidylcholine vesicles. We first describe the design and the syntheses of the conjugates. Then, we use UV−Vis absorption, steady-state fluorescence emission, electron microscopy, and fluorescence correlation spectroscopy after two-photon excitation to show that these DNA-lipids form spherical micelles in aqueous solution and incorporate much better in micelles than in vesicles. We also investigate the significance of the lipophilic chains of these DNA-lipids on the melting behavior of the double-stranded hybrids: in water melting curves are broadened whereas in amphiphilic assemblies duplexes melt as the unconjugated controls. This work is expected to be useful for improving the rational design of antisense medicines.