posted on 2004-05-20, 00:00authored byC. Gosse, A. Boutorine, I. Aujard, M. Chami, A. Kononov, E. Cogné-Laage, J.-F. Allemand, J. Li, L. Jullien
This report examines the organization properties of new fluorescent DNA-lipids, either alone in water or in
interaction with 1-octyl-β-d-glucopyranoside micelles or egg phosphatidylcholine vesicles. We first describe
the design and the syntheses of the conjugates. Then, we use UV−Vis absorption, steady-state fluorescence
emission, electron microscopy, and fluorescence correlation spectroscopy after two-photon excitation to show
that these DNA-lipids form spherical micelles in aqueous solution and incorporate much better in micelles
than in vesicles. We also investigate the significance of the lipophilic chains of these DNA-lipids on the
melting behavior of the double-stranded hybrids: in water melting curves are broadened whereas in amphiphilic
assemblies duplexes melt as the unconjugated controls. This work is expected to be useful for improving the
rational design of antisense medicines.