posted on 2020-02-07, 21:08authored byAlberto
G. Costa, Nídia C. Yoshida, Walmir S. Garcez, Renata T. Perdomo, Maria de Fátima
C. Matos, Fernanda R. Garcez
Propolis samples collected from five
areas in Mato Grosso do Sul
state, Midwest Brazil, comprising portions of the Cerrado, Pantanal,
and Atlantic Forest ecosystems, were investigated for metabolomic
profiles and evaluated for antioxidant and antitumor potential. Chemical
profiles were determined by HPLC-DAD-MS/MS data and evaluated using
principal component analysis and hierarchical clustering analysis
to discern chemical composition patterns. Based on phytogeographical
origin and chemical composition, 20 potential markers were identified
and five groups were distinguished: (I) Cerrado/Central, (II) Atlantic
Forest/South, (III) Cerrado–Pantanal transition area/Northwest,
(IV) Cerrado/North, and (V) Pantanal/West. Drawing on HPLC-DAD-MS/MS
and NMR data, 47 compounds were successfully or tentatively identified,
including prenylated phenylpropanoids, flavonoids, isoflavonoids,
and di- and triterpenoids, among other constituents. Isoflavonoids,
typically found in red propolis from Northeast Brazil, are being reported
for the first time in a propolis sample from the Midwest. A new prenylated
aromatic compound, (E)-3-[4-hydroxy-3-(2-hydroxy-3-methylbut-3-en-1-yl)-5-(3-methylbut-2-en-1-yl)phenyl]propenoic
acid, was obtained. Samples in group II exhibited promising antitumor
potential against prostate and breast carcinoma cells, as did samples
in groups III and IV against the latter cell line. The sample in group
I, despite containing the highest amount of total phenolic compounds
and being the only sample to exhibit scavenging activity against DPPH,
was not the most cytotoxic against the cell lines tested.