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Metabolic Profiling of Different Parts of Acer truncatum from the Mongolian Plateau Using UPLC-QTOF-MS with Comparative Bioactivity Assays
journal contribution
posted on 2019-01-23, 22:14 authored by Ronghui Gu, Levi Rybalov, Adam Negrin, Taylan Morcol, Weiwen Long, Amanda K. Myers, Giorgis Isaac, Jimmy Yuk, Edward J. Kennelly, Chunlin LongAcer truncatum is an important ornamental, edible,
and medicinal plant resource in China. Previous phytochemical research
has focused on the leaf (AL) due to its long history as a tea for
health. Other parts such as the branch (ABr), bark (ABa), fruit (AF),
and root (AR) have drawn little attention regarding their metabolites
and bioactivities. The strategy of an in-house chemical library combined
with Progenesis QI informatics platform was applied to characterize
the metabolites. A total of 98 compounds were characterized or tentatively
identified, including 63 compounds reported from this species for
the first time. Principal component analysis showed the close clustering
of ABr, ABa, and AR, indicating that they share similar chemical components,
while AL and AF clustered more distantly. By multiple orthogonal partial
least-squares discriminant analyses (OPLS-DA), 52 compounds were identified
as potential marker compounds differentiating these different plant
parts. The variable influence on projection score from OPLS-DA revealed
that catechin, procyanidins B2 or B3, and procyanidins C1 or C2 are
the significant metabolites in ABa extracts, which likely contribute
to its antioxidant and cytotoxic activities.
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Comparative Bioactivity Assays Acer truncatum63 compoundsmetaboliteABa extractsplant resourceDifferent Partsprocyanidins B 2marker compoundsPrincipal component analysischemical librarychemical componentsAcer truncatumPrevious phytochemical researchcytotoxic activitiesleast-squares discriminant analysesprocyanidins C 1plant partsOPLS-DAC 2Other partsprojection scoreMetabolic Profiling98 compoundsAFALARProgenesis QI informatics platformMongolian PlateauUPLC-QTOF-MSABr
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