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Merocyanine Dyes with Improved Photostability

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journal contribution
posted on 19.07.2007, 00:00 by Alexei Toutchkine, Dan-Vinh Nguyen, Klaus M. Hahn
Merocyanine dyes have proven valuable for live cell fluorescence imaging applications, but many structures have been limited by rapid photobleaching. We show that photostability is substantially enhanced for merocyanines having a cyano group at a specific position in the central polymethine chain. Evidence is presented that this is due to reduction in reactivity of the dyes with singlet oxygen. These results point toward cyano-substitution as a general strategy for improving dye performance in imaging applications.