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Matrix-Matched Calibration Curves for Assessing Analytical Figures of Merit in Quantitative Proteomics
journal contribution
posted on 2020-02-24, 13:08 authored by Lindsay
K. Pino, Brian C. Searle, Han-Yin Yang, Andrew N. Hoofnagle, William S. Noble, Michael J. MacCossMass
spectrometry is a powerful tool for quantifying protein abundance
in complex samples. Advances in sample preparation and the development
of data-independent acquisition (DIA) mass spectrometry approaches
have increased the number of peptides and proteins measured per sample.
Here, we present a series of experiments demonstrating how to assess
whether a peptide measurement is quantitative by mass spectrometry.
Our results demonstrate that increasing the number of detected peptides
in a proteomics experiment does not necessarily result in increased
numbers of peptides that can be measured quantitatively.