jp510980b_si_001.pdf (718.32 kB)

Master and Slave Relationship Between Two Types of Self-Propagating Insulin Amyloid Fibrils

Download (718.32 kB)
journal contribution
posted on 26.11.2014, 00:00 by Weronika Surmacz-Chwedoruk, Viktoria Babenko, Wojciech Dzwolak
Cross-seeding of fibrils of bovine insulin (BI) and LysB31-ArgB32 human insulin analog (KR) induces self-propagating amyloid variants with infrared features inherited from mother seeds. Here we report that when native insulin (BI or KR) is simultaneously seeded with mixture of equal amounts of both templates (i.e., of separately grown fibrils of BI and KR), the phenotype of resulting daughter fibrils is as in the case of the purely homologous seeding: heterologous cotemplates accelerate the fibrillation but do not determine infrared traits of the daughter amyloid. This implies that fibrillation-promoting and structure-imprinting properties of heterologous seeds become uncoupled in the presence of homologous seeds. We argue that explanation of such behavior requires that insulin molecules partly transformed through interactions with heterologous fibrils are subsequently recruited by homologous seeds. The selection bias toward homologous daughter amyloid is exceptional: more than 200-fold excess of heterologous seed is required to imprint its structural phenotype upon mixed seeding. Our study captures a snapshot of elusive docking interactions in statu nascendi of elongation of amyloid fibril and suggests that different types of seeds may collaborate in sequential processing of soluble protein into fibrils.

History

Exports